Objective The aim of this study was to investigate the time course of changes of serum testosterone (T), cortisol (C), dehydroepiandrosterone sulfate (DHEA-S), insulin and blood glucose during two-week taper, immediately after match, and two-week recovery in elite male taekwondo athletes. Methodology Eight male taekwondo athletes competing at national level (age: 20.9 ± 0.3 yrs, BMI: 22.0 ± 0.7 kg/m2) participated in this study. The fasting blood samples were collected at two weeks before match (BM), one day after match (AM) and 2 weeks post-match recovery (PM). The blood glucose was evaluated using portable glucose meter, and the serum levels of insulin, testosterone (T), cortisol (C), and dehydroepiandrosterone sulfate (DHEA-S, D) were measured using commercial ELISA kits. The insulin and blood glucose were used to calculate the homeostatic model assessment of insulin resistance index (HOMA-IR index). Results & Discussion The T/C ratio (AM: 0.24 ± 0.01, PM: 0.30 ± 0.03) and HOMA-IR (AM: 1.90 ± 0.21, PM: 1.44 ± 0.17) were significantly lower at AM than PM (p < 0.05) but the T/C ratio, D/C ration and HOMA-IR were no difference between BM and AM. The physiological stress status, reflecting by the T/C and D/C ratios, were comparable between tapering and competitive periods, whereas the stress level was rapidly declined at 2-weeks post-competitive recovery. In addition, the insulin sensitivity appeared at impaired state before and during match period, but this situation would improve after two weeks recovery. Conclusion We demonstrated, that, in the elite male Taekwondo athletes, the physiological stress status and insulin resistance index were comparable between tapering and competitive periods, whereas the stress level was markedly lower and insulin sensitivity was improved at 2-weeks post-competitive recovery.